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Key words: SigmaPlot SPSS, sigmaplot, spss
SigmaPlot SPSS Enzyme Kinetics Module - SigmaPlot Module
Simplified Data Management to Organize Your Data and Results
Fast analysis starts with the module's Data Entry Wizard. The Data Entry Wizard provides streamlined data entry into the SigmaPlot worksheet. It automatically determines the data column assignments based on
the study type you select and makes setting up your data in the worksheet a breeze. Cut and paste data from any application or enter your data manually. The module allows you to select the units of your velocity or
other dependent variable values from a drop down list. These units are carried to the worksheet and to the final graphs. Replicate velocities may be entered, and for inhibition studies, you can choose to vary either
the substrate or the inhibitor concentrations for each series. Select from lists of unit values for substrate and velocity or enter your own; the units are carried through to the reports and graphs. And once you run
your analysis, organize your reports, graphs and equations in the SigmaPlot Notebook.
Select From a Wide Range of Built-In Models
Select from multiple equation groups to analyze your data. The module provides about 40 different built-in equations to characterize different types of reaction mechanisms. It automatically estimates the
initial parameters for the selected model(s), and uses the Marquardt-Levenberg algorithm to determine the parameter values. You get a detailed statistical report as well as a data report that
includes the Km, Ki and Vmax values and their standard errors. You can simultaneously fit and compare multiple models and graphs from library of built
-in equations. You can also enter your own custom equations and save them for future use.
Easily Determine the Best-Fit Inhibition Model for Your Data
To compare the different inhibition models, the Enzyme Kinetics Module provides a detailed report with complete statistical analysis for each model, as well as provides information on comparing the different
models. Several goodness of fit criteria including Akaike's AIC are provided to determine the best model. Statistical results include parameter value confidence intervals, a residual runs test and outlier
detection. The data report includes replicate mean velocities and their standard errors, the predicted and residual values from the curve fit and an indication of data outliers. The statistical report
generated for each model includes the parameter values with their standard errors and 95% confidence intervals, Akaike, R2, sum of squares, standard error of the regression, and the
significance level for the runs test. The selected models can be automatically ranked by goodness of fit.
See Results Clearly With Interactive Graphs
You can create any one or all of the built-in graphs provided for each study. The module automatically generates interactive graphs you choose to display your results. You can modify the graphs as desired to
present your results exactly the way you want to using SigmaPlot's ability to customize your graphs. Many of the graph algorithms automatically determine where fit lines intersect the axes and present
the graphs in this manner. To help determine the type of inhibition, the curve fit lines are designed to allow you to extend the graph axes to show fit line intersection.
Publish Your Work Anywhere
The Enzyme Kinetics Module runs seamlessly with SigmaPlot. You can easily create custom reports of your results including tables and graphs, incorporate your graphs into presentations or
export your graphs to a wide range of graphic file formats to send it for publishing in journals. You can also create an HTML output of your graph and report to disseminate on the WWW.
Enzyme Kinetic Analysis Doesn't Get Any Easier
The Enzyme Kinetics Module is an add-on to SigmaPlot 2000 that provides the curve fitting and graphing capabilities you need to analyze and present your enzyme kinetics data - quickly and easily. The
module follows SigmaPlot's tradition of award-winning interface, ease-of-use, and intelligent wizards to guide you through your entire analysis. Just import or enter your data, select the type of
study and the equation you would like to fit, as well as the interactive graphs to display your results. Using SigmaPlot's powerful nonlinear curve-fitter, the module fits the
selected equations to your data, as well as provides interactive graphs you need to see to study the kinetics mechanism. What's more, you get a detailed
report complete with all statistical parameters for each model you fit so you can easily compare the different models to identify the best one for your data. It's that easy!
Plus, the Power of SigmaPlot
In addition to specialized analysis and graphs the Enzyme Kinetics Module provides, you have access to all of SigmaPlot's analytical, graphing and
presentation capabilities! You can get started and publish your results faster than you ever imagined. And, SigmaPlot provides the flexibility you need to
customize your graph and present your results exactly the way you want to. In fact, SigmaPlot's graphing flexibility has inspired the readers of Scientific
Computing and Instrumentation to award SigmaPlot their prestigious Reader's Choice Award eight years running!
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